Emphasizing the Toxicity of Heavy Metal Ions on Plants: Monitoring with an Amino Acids Assay

Authors

  • Olga PINTILIE Al. I. Cuza University of Iaşi, Romania
  • Marius ZAHARIA Al. I. Cuza University of Iaşi, Romania
  • Lucia TUDORACHI Al. I. Cuza University of Iaşi, Romania
  • Sabina BĂNCILĂ Al. I. Cuza University of Iaşi, Romania
  • Gabi DROCHIOIU Al. I. Cuza University of Iaşi, Romania
  • Ion SANDU Al. I. Cuza University of Iaşi, Romania

Keywords:

biostructure, determining amino acids, hypoxia, germination tests, heavy metals, ninhydrin reagent

Abstract

In order to emphasize modifications on living organisms resulted from interaction with heavy metals, we applied a simple, sensitive, quick, inexpensive and reproducible method. It evaluates modifications through determining released amino acids. This method comprises three stages: treating plants with the investigated heavy metals, extracting resulted amino acids and dosing them with a ninhydrin reagent. The colored solution resulted from ninhydrin dosing is spectrophotometrically measured at 516 nm. The Lambert-Beer law is valid within the concentration field of 0-20 μg mL–1.

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Published

2014-03-15

Issue

Vol. 37 No. 1 (2014)

Section

Articles

How to Cite

1.
Emphasizing the Toxicity of Heavy Metal Ions on Plants: Monitoring with an Amino Acids Assay. Ann_UGAL_MMS [Internet]. 2014 Mar. 15 [cited 2026 Nov. 2];37(1):51-6. Available from: https://ojs.ugal.ro/index.php/mms/article/view/2647

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