Industrial nutrient medium use for yeast selenium preparation

Résumé

The paper presents the preparation of selenium yeast from spent brewer’s yeast by using industrial nutrient medium (malt wort and sparge water). The chemical treatment of the yeast biomass was done with sodium-selenite solution. The preparation of selenium – enriched Saccharomyces uvarum in different conditions of temperature, time, yeast biomass proportion and sodium-selenite concentration of the medium was analysed. Using a culture medium supplemented with 30–180 μg/ ml sodium-selenite results in total selenium accumulation in the range of 0.6–2.2 mg/g for malt wort and 0.4–1.6 mg/g for sparge water and in organic selenium accumulation in the range of 0.6–1.5 mg/g for malt wort and 0.3–0.9 mg/g for sparge water measured by ICP- MS method.