Biomass and pigments production of the mixed culture of microalgae (Hyaloraphidium contortum and Chlorella vulgaris) by cultivation in media based on commercial fertilizer

Abstract

For the mixed culture of Chlorella vulgaris and Hyaloraphidium contortum, was determined the effect of different sources of nutrients (Nitrofoska ®, Poliverdol ® and Guillard) on the production of photosynthetic pigments and biomass. Bioassays were performed in quadruplicate in batch cultures maintained in aeration and continuous illumination. The growth was determined by cell counting and the production of pigment by spectrophotometry. The cell density increased with time of planting, obtaining maximum production at 24 days with an average of 1.38 104±
5603.94; 1.09 104 ± 29.125 and 3.04 104± 8.900 cells mL-1 in Nitrofoska®, Poliverdol® and Guillard, respectively. The pigment (chlorophyll a and b and carotenoids) production was moderated (1.11 μg mL-1 at 24 days) in the medium Guillard during all-time research. Commercial agricultural fertilizers are an alternative source of nutrients for growth and pigment production in the mixed culture of microalgae.