Extraction and antioxidant activities assay of polysaccharides from white Hyacinth bean and promoting-growth to probiotics

Abstract

The extraction parameters of water-soluble polysaccharides (WPs) from white hyacinth bean were optimized using single-factor and orthogonal experiment. The antioxidant activities of WPs were presented by assaying three different radicals, 2,2-diphenyl-1-picrylhydrazy radical (DPPH radical), hydroxyl radical and superoxide radical scavenging ability. In addition, the effects of WPs obtained on the growth of three probiotic strains (Lactobacillus acidophilus LA5, Bifidobacterium bifidum BB01 and Lactobacillus bulgaricus LB6) were also determined by measuring the OD and pH value of culture medium. According to the results, the optimum extraction parameters were as follows: the ratio of water to material was 50, extraction time was 2h and the extraction temperature was 95°C. The yield of WPs reached 1.15±0.07% under this condition. In addition, the WPs had different scavenging ability on three radicals (hydroxyl > DPPH > superoxide). And the WPs could promote the growth of LA5, BB01 and LB6.