Safety and protective effect of bifidobactérium spp. used as probiotic agent in vivo against enteropathogenic escherichia coli

Résumé

This study showed the effect of fermented milk with Bifidobacterium bifidum on the contaminations by Escherichia coli enteropathogenic EPEC and intestinal disorders. The B. bifidum strain (Bf) was isolated on MRS medium containing 0.5g/L of cysteine hydrochloride and 2 mg/L of nalidixic acid. This strain was isolated from breastfed infant feces. This strain is considered as a model strain, for antagonism in vivo developed in the rat (wistar). It has has been demonstrated that the administration of Bifidobacterium strain cause a significant reduction of the rate of E. coli enteropathogenic multiplication in the rats feces. Results of the macroscopic study of histological sections have confirmed the positive effect of treatment with bifidobacteria. In this study, the effect of Bifidobacterium addition in reducing the enteropathogenic bacteria effect in the digestive tract was confirmed by in vitro and in vivo conditions.