Aspects of antibiotic resistance for Klebsiella Pneumoniae in a children hospital

Abstract

This study showed the resistance profile of Klebsiella pneumoniae strains which were isolated between January 2018 - November 2021, from various samples at Emergency Clinical Hospital for Children “St. Ioan” in Galati, Romania. Identification of K. pneumoniae isolates was achieved by analyzing colony morphology, microscopic examination, and by performing biochemical tests and automatic methods using Vitek 2 system. Testing of antibiotics susceptibility was done by using the disk diffusion method and by determining the minimum inhibitory concentration (MIC). Further, 133 strains were identified, the most of them were isolated from the respiratory samples (74 strains representing 57.31%). Additional, 80 strains (60.15%) were isolated from patients under one year of age, 68 isolates (51.13%) from female patients, 72 isolates (54.14%) from patients living in urban areas. More than 80% of K. pneumoniae strains were susceptible to carbapenems, fosfomycin and amikacin while over 70% were susceptible to ciprofloxacin, nitrofurantoin and gentamicin. Over 42% of the isolates were multidrug resistant (MDR). Antimicrobial resistance in K. pneumoniae is a real threat that requires vigilant monitorization. This study revealed a low rate of resistance to carbapenems, fosfomycin and amikacin.