Study of preliminary biotechnological conditions for Pleurotus ostreatus cultivation on submerged system

Abstract

The goal of this work was to identify the biotechnogical conditions (i.e. inoculum concentration, nutrional sources (carbon and nitrogen), pH and time of cultivation) for improving the yield of biomass production of Pleurotus ostreatus by cultivation on submerged culture conditions. The submerged cultivation was done in Erlenmeyer vessels in rotary shaker with controlled temperature, for 10 days at 26°C and 150 rpm. Four concentrations of inoculum 0.2%, 5.0%, 7.5% and 10.0%, and also three values of pH (5.0, 5.5 and 6.0) were tested. Also, it was tested the qualitative effect of some carbon and nitrogen sources for an optimal growth and multiplication of mushroom in liquid medium, in submerged conditions with agitation. The best Pleurotus ostreatus biomass yield (7.58 g dry biomass ·L^-1) was obtained with 0.2% inoculum concentration when dry biomass yields increased with 30%. By using dextrose in concentration of 40 g/L and peptone and yeast extract (1:1) in concentration of 5g/L have a positive influence of mycelium growth and biomass forming. These preliminary results will open new further studies on mushroom submerged cultivation.