Use of sesame oil cake for lipase production from a newly marine isolated Bacillus sonorensis

Abstract

A Gram positive, motile, mesophilic marine Bacillus sonorensis strain capable of producing extracellular lipase was isolated from marine clams, Paphia malabarica, collected from Kalbhadevi estuary, India. The efficiency of sesame oil cake as a substrate for lipase production by marine Bacillus sonorensis strain in submerged fermentation (SmF) has been studied and reported. The sesame oil cake was selected as it is rich in protein, cheap, abundantly available being an agroindustrial waste and can be constructively used as one of the main constituent in medium formulation. Different physical and chemical parameters such as pH, temperature, and substrate concentration and incubation time were optimized. The lipase activity was found to be maximum when pH of medium containing 4% of mustard oil cake was 5.0, at 40°C, after 48 hrs of submerged cultivation. Further, the growth curve studies indicated that the production of extracellular lipase was initiated in the early lag phase and was maximum in the late exponential phase.